Pic50 to Ic50 Calculator

pIC50 to IC50 Converter

function calculateIC50() { var pic50Input = document.getElementById("pic50Value").value; var pic50 = parseFloat(pic50Input); var resultDiv = document.getElementById("result"); if (isNaN(pic50)) { resultDiv.innerHTML = "Please enter a valid numeric pIC50 value."; return; } // IC50 = 10^(-pIC50) in Molar (M) var ic50Molar = Math.pow(10, -pic50); // Convert to more common units: nanomolar (nM) and micromolar (µM) var ic50NanoMolar = ic50Molar * 1e9; // 1 M = 10^9 nM var ic50MicroMolar = ic50Molar * 1e6; // 1 M = 10^6 µM resultDiv.innerHTML = "

Calculated IC50:

" + "IC50 (Molar): " + ic50Molar.toExponential(3) + " M" + "IC50 (Nanomolar): " + ic50NanoMolar.toFixed(3) + " nM" + "IC50 (Micromolar): " + ic50MicroMolar.toFixed(3) + " µM"; }

Understanding pIC50 and IC50 in Pharmacology

In pharmacology and biochemistry, IC50 (half maximal inhibitory concentration) and pIC50 are crucial metrics used to quantify the potency of a substance in inhibiting a specific biological or biochemical function. This calculator helps you quickly convert between these two commonly used values.

What is IC50?

The IC50 value represents the concentration of an inhibitor (e.g., a drug or compound) required to inhibit a given biological process or component by 50%. It is a direct measure of the potency of an antagonist or inhibitor. A lower IC50 value indicates a more potent inhibitor, meaning less of the compound is needed to achieve 50% inhibition.

IC50 values are typically expressed in molar units (M, µM, nM, pM) and are derived from dose-response curves, which plot the percentage of inhibition against the logarithm of the inhibitor concentration.

What is pIC50?

The pIC50 is the negative logarithm (base 10) of the IC50 value. It is calculated using the formula:

pIC50 = -log₁₀(IC50)

The primary reason for using pIC50 is to express IC50 values on a more convenient, positive, and linear scale, especially when dealing with very small molar concentrations. For example, an IC50 of 100 nM (1 x 10^-7 M) becomes a pIC50 of 7.0. A higher pIC50 value indicates a more potent inhibitor.

Why Convert Between pIC50 and IC50?

• Data Presentation: pIC50 values are often preferred for graphical representation and statistical analysis because they provide a linear scale where differences in potency are more easily visualized.
• Ease of Comparison: Comparing compounds with pIC50 values is often more intuitive. For instance, a compound with pIC50 = 8 is clearly more potent than one with pIC50 = 7.
• Standardization: Many scientific databases and publications report pIC50 values, making conversions necessary for researchers working with different data formats.

How the Conversion Works

The conversion from pIC50 back to IC50 (in Molar) is straightforward:

IC50 (M) = 10^-pIC50

Once you have the IC50 in Molar, you can easily convert it to nanomolar (nM) or micromolar (µM) for practical use:

• 1 M = 1,000,000 µM (10⁶ µM)
• 1 M = 1,000,000,000 nM (10⁹ nM)

Examples:

• Example 1: If a compound has a pIC50 of 7.0, its IC50 would be 10^-7.0 M, which is 0.0000001 M or 100 nM.
• Example 2: A highly potent compound with a pIC50 of 9.3 would have an IC50 of 10^-9.3 M, which is approximately 5.01 x 10^-10 M or 0.501 nM.
• Example 3: A less potent compound with a pIC50 of 5.5 would have an IC50 of 10^-5.5 M, which is approximately 3.16 x 10^-6 M or 3.16 µM.

Use the calculator above to perform these conversions quickly and accurately for your research or studies.